Vol. 11, No.1, pp.81-86 (2017)
Influence of Pulsed Electric Field to Leaf Lettuce Evaluated on Chlorophyll Fluorescence Measurement Using Pulsed-Amplitude-Modulated Fluorometer
T. Sonoda1, Y. Higashi2, Y. Yamada1, D. Wang2, T. Namihira2, and H. Akiyama2
1Graduate School of Science and Technology, Kumamoto University, Japan
2Institute of Pulsed Power Science, Kumamoto University, Japan
Abstract
Due to expectations to apply pulsed power technologies for various fields, applications of a pulsed electric field (PEF) on biological cells have been actively studied. In addition, the technique of chlorophyll fluorescence has become ubiquitous to reveal photosynthetic mechanism or stress reactions of photosynthetic organisms in ecophysiology studies in recent years. In this paper, chlorophyll fluorescence measurement was employed to reveal PEF effects to plants. Leaf lettuce was chosen as the materials of this experimental study and the subjects were treated with relatively low intensity of PEF, 0.2 kV/cm and relatively high intensity of PEF, 1.0 kV/cm, with 400 ns and 500 pulse. Experimental results using pulsed-amplitude-modulated fluorometer (PAM) showed that 0.2 kV/cm has increased photosynthetic electron transport rate (ETR), and photochemical quenching (qP) that are related to redox state of electron acceptor in photosystem II. On the other hands, 1.0 kV/cm has decreased non-photochemical quenching (NPQ) that are related to heat dissipation of light energy.
Keywords - Pulsed electric field (PEF), leaf lettuce, chlorophyll fluorescence, PAM