Evaluation of Reactive Oxygen Species in Non-thermal Plasma-treated Water
Evaluation of Reactive Oxygen Species in Non-thermal Plasma-treated Water
K. Sano1, H. Kurita1, T. Nakajima1, M. Shimizu1, M. Kuzumoto2, H. Yasuda1, K. Takashima1, and A. Mizuno1
1Department of Environmental and Life Sciences, Toyohashi University of Technology, Japan
2Power Electronics Technology Laboratory, Advanced Technology R&D Center, Mitsubishi Electric Corporation, Japan
Abstract- Non-thermal atmospheric pressure plasma for biological applications has been notably developed in recent years. Among them, chemically active species, such as reactive oxygen species (ROSs) generated in water using the plasma play an important role in inactivation of biomolecules. Here we measured the intensity of ROSs by electron spin resonance (ESR) spectroscopy with spin trapping technique to assay the ROSs effects comparing DNA strand breaks. The correlation between the signal intensity of OH radical measured by ESR and the number of strand breaks obtained by single-molecule DNA observation is discussed.
Keywords- Non-thermal atmospheric pressure plasma, reactive oxygen species, electron spin resonance